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rabbit anti p2x3  (Neuromics)


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    Structured Review

    Neuromics rabbit anti p2x3
    Rabbit Anti P2x3, supplied by Neuromics, used in various techniques. Bioz Stars score: 93/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti-p2x3/P2X3/10__21203_slash_rs__3__rs___7198392_slash_v1-194-16-19
    Average 93 stars, based on 126 article reviews
    rabbit anti p2x3 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Alteration of dorsal root ganglion P2X3 receptor expression and function following spinal nerve ligation in the rat.
    Article Snippet: One subtype of ATP-gated ion channel, the P2X3 receptor, is expressed primarily on peripheral sensory neurons.. While it is known that P2X3 receptors can participate in certain forms of nociceptive signaling, their involvement in neuropathic pain transmission is not known.. We have examined the expression and function of P2X3 receptors in a rat spinal nerve ligation model of neuropathic pain.

    Article Title: A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neurons
    Article Snippet: After being blocked for 1 h at 37°C in PBS containing 10% goat serum and 0.3% Triton X-100, the sections were incubated with rabbit anti-NF200 (1:500, Sigma-Aldrich, St. Louis, MO) 48 , rabbit anti-P2X3 (1:500, Neuromics, Edina, MN) 48 , Biotinylated IB4 (1:100, Sigma) 48 , rabbit anti-CGRP (1:500, EMD, Billerica, MA) 48 , mouse anti-NeuN (1:600, EMD) 48 , mouse anti-GFAP (1:500, Sigma) 48 , or mouse anti-OX-42 (1:400, Sigma) 48 overnight at 4°C.

    Article Title: The behavioral and neuroanatomical effects of IB4-saporin treatment in rat models of nociceptive and neuropathic pain.
    Article Snippet: One distinguishing feature of primary afferent neurons is their ability to bind the lectin IB4.. Previous work suggested that neurons in the inner part of lamina II (IIi), onto which IB4-positive sensory neurons project, facilitate nociceptive transmission following tissue or nerve injury.. Using an IB4-saporin conjugate (IB4-SAP), we examined the contribution of IB4-positive neurons to nociceptive processing in rats with and without nerve injury.

    Article Title: Low-Threshold Mechanosensitive VGLUT3-Lineage Sensory Neurons Mediate Spinal Inhibition of Itch by Touch
    Article Snippet: Skin sections from Vglut3-cre / TdTomato mice were blocked with 5% donkey serum and 0.2% Triton X-100 in PBS, then immunostained with rabbit anti-PGP9.5 (1:200; EMD Millipore), rabbit anti-calcitonin gene-related peptide (CGRP) (1:300; Peninsula Laboratories), rabbit anti-P2X3 (1:200; Neuromics), or rabbit anti-Neurofilament H (NFH) antibody (1:200, Sigma Millipore) at 4°C overnight, followed by incubation with the corresponding secondary antibody conjugated with AlexaFluor-488 (1:300) at 37°C for 30 min. For immunostaining of VGLUT3, skin was dissected from Vglut3-cre / TdTomato mice and frozen in optimal cutting temperature compound (Tissue-Tek), and cut in 40 μm sections on a cryostat.

    Immunostaining:

    Article Title: Alteration of dorsal root ganglion P2X3 receptor expression and function following spinal nerve ligation in the rat.
    Article Snippet: One subtype of ATP-gated ion channel, the P2X3 receptor, is expressed primarily on peripheral sensory neurons.. While it is known that P2X3 receptors can participate in certain forms of nociceptive signaling, their involvement in neuropathic pain transmission is not known.. We have examined the expression and function of P2X3 receptors in a rat spinal nerve ligation model of neuropathic pain.

    Article Title: A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neurons
    Article Snippet: After being blocked for 1 h at 37°C in PBS containing 10% goat serum and 0.3% Triton X-100, the sections were incubated with rabbit anti-NF200 (1:500, Sigma-Aldrich, St. Louis, MO) 48 , rabbit anti-P2X3 (1:500, Neuromics, Edina, MN) 48 , Biotinylated IB4 (1:100, Sigma) 48 , rabbit anti-CGRP (1:500, EMD, Billerica, MA) 48 , mouse anti-NeuN (1:600, EMD) 48 , mouse anti-GFAP (1:500, Sigma) 48 , or mouse anti-OX-42 (1:400, Sigma) 48 overnight at 4°C.

    Article Title: The behavioral and neuroanatomical effects of IB4-saporin treatment in rat models of nociceptive and neuropathic pain.
    Article Snippet: One distinguishing feature of primary afferent neurons is their ability to bind the lectin IB4.. Previous work suggested that neurons in the inner part of lamina II (IIi), onto which IB4-positive sensory neurons project, facilitate nociceptive transmission following tissue or nerve injury.. Using an IB4-saporin conjugate (IB4-SAP), we examined the contribution of IB4-positive neurons to nociceptive processing in rats with and without nerve injury.

    Article Title: Low-Threshold Mechanosensitive VGLUT3-Lineage Sensory Neurons Mediate Spinal Inhibition of Itch by Touch
    Article Snippet: Skin sections from Vglut3-cre / TdTomato mice were blocked with 5% donkey serum and 0.2% Triton X-100 in PBS, then immunostained with rabbit anti-PGP9.5 (1:200; EMD Millipore), rabbit anti-calcitonin gene-related peptide (CGRP) (1:300; Peninsula Laboratories), rabbit anti-P2X3 (1:200; Neuromics), or rabbit anti-Neurofilament H (NFH) antibody (1:200, Sigma Millipore) at 4°C overnight, followed by incubation with the corresponding secondary antibody conjugated with AlexaFluor-488 (1:300) at 37°C for 30 min. For immunostaining of VGLUT3, skin was dissected from Vglut3-cre / TdTomato mice and frozen in optimal cutting temperature compound (Tissue-Tek), and cut in 40 μm sections on a cryostat.

    Immunohistochemistry:

    Article Title: Alteration of dorsal root ganglion P2X3 receptor expression and function following spinal nerve ligation in the rat.
    Article Snippet: One subtype of ATP-gated ion channel, the P2X3 receptor, is expressed primarily on peripheral sensory neurons.. While it is known that P2X3 receptors can participate in certain forms of nociceptive signaling, their involvement in neuropathic pain transmission is not known.. We have examined the expression and function of P2X3 receptors in a rat spinal nerve ligation model of neuropathic pain.

    Article Title: A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neurons
    Article Snippet: After being blocked for 1 h at 37°C in PBS containing 10% goat serum and 0.3% Triton X-100, the sections were incubated with rabbit anti-NF200 (1:500, Sigma-Aldrich, St. Louis, MO) 48 , rabbit anti-P2X3 (1:500, Neuromics, Edina, MN) 48 , Biotinylated IB4 (1:100, Sigma) 48 , rabbit anti-CGRP (1:500, EMD, Billerica, MA) 48 , mouse anti-NeuN (1:600, EMD) 48 , mouse anti-GFAP (1:500, Sigma) 48 , or mouse anti-OX-42 (1:400, Sigma) 48 overnight at 4°C.

    Article Title: The behavioral and neuroanatomical effects of IB4-saporin treatment in rat models of nociceptive and neuropathic pain.
    Article Snippet: One distinguishing feature of primary afferent neurons is their ability to bind the lectin IB4.. Previous work suggested that neurons in the inner part of lamina II (IIi), onto which IB4-positive sensory neurons project, facilitate nociceptive transmission following tissue or nerve injury.. Using an IB4-saporin conjugate (IB4-SAP), we examined the contribution of IB4-positive neurons to nociceptive processing in rats with and without nerve injury.

    Article Title: Low-Threshold Mechanosensitive VGLUT3-Lineage Sensory Neurons Mediate Spinal Inhibition of Itch by Touch
    Article Snippet: Skin sections from Vglut3-cre / TdTomato mice were blocked with 5% donkey serum and 0.2% Triton X-100 in PBS, then immunostained with rabbit anti-PGP9.5 (1:200; EMD Millipore), rabbit anti-calcitonin gene-related peptide (CGRP) (1:300; Peninsula Laboratories), rabbit anti-P2X3 (1:200; Neuromics), or rabbit anti-Neurofilament H (NFH) antibody (1:200, Sigma Millipore) at 4°C overnight, followed by incubation with the corresponding secondary antibody conjugated with AlexaFluor-488 (1:300) at 37°C for 30 min. For immunostaining of VGLUT3, skin was dissected from Vglut3-cre / TdTomato mice and frozen in optimal cutting temperature compound (Tissue-Tek), and cut in 40 μm sections on a cryostat.

    Blocking Assay:

    Article Title: Alteration of dorsal root ganglion P2X3 receptor expression and function following spinal nerve ligation in the rat.
    Article Snippet: One subtype of ATP-gated ion channel, the P2X3 receptor, is expressed primarily on peripheral sensory neurons.. While it is known that P2X3 receptors can participate in certain forms of nociceptive signaling, their involvement in neuropathic pain transmission is not known.. We have examined the expression and function of P2X3 receptors in a rat spinal nerve ligation model of neuropathic pain.

    Article Title: A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neurons
    Article Snippet: After being blocked for 1 h at 37°C in PBS containing 10% goat serum and 0.3% Triton X-100, the sections were incubated with rabbit anti-NF200 (1:500, Sigma-Aldrich, St. Louis, MO) 48 , rabbit anti-P2X3 (1:500, Neuromics, Edina, MN) 48 , Biotinylated IB4 (1:100, Sigma) 48 , rabbit anti-CGRP (1:500, EMD, Billerica, MA) 48 , mouse anti-NeuN (1:600, EMD) 48 , mouse anti-GFAP (1:500, Sigma) 48 , or mouse anti-OX-42 (1:400, Sigma) 48 overnight at 4°C.

    Article Title: The behavioral and neuroanatomical effects of IB4-saporin treatment in rat models of nociceptive and neuropathic pain.
    Article Snippet: One distinguishing feature of primary afferent neurons is their ability to bind the lectin IB4.. Previous work suggested that neurons in the inner part of lamina II (IIi), onto which IB4-positive sensory neurons project, facilitate nociceptive transmission following tissue or nerve injury.. Using an IB4-saporin conjugate (IB4-SAP), we examined the contribution of IB4-positive neurons to nociceptive processing in rats with and without nerve injury.

    Article Title: Low-Threshold Mechanosensitive VGLUT3-Lineage Sensory Neurons Mediate Spinal Inhibition of Itch by Touch
    Article Snippet: Skin sections from Vglut3-cre / TdTomato mice were blocked with 5% donkey serum and 0.2% Triton X-100 in PBS, then immunostained with rabbit anti-PGP9.5 (1:200; EMD Millipore), rabbit anti-calcitonin gene-related peptide (CGRP) (1:300; Peninsula Laboratories), rabbit anti-P2X3 (1:200; Neuromics), or rabbit anti-Neurofilament H (NFH) antibody (1:200, Sigma Millipore) at 4°C overnight, followed by incubation with the corresponding secondary antibody conjugated with AlexaFluor-488 (1:300) at 37°C for 30 min. For immunostaining of VGLUT3, skin was dissected from Vglut3-cre / TdTomato mice and frozen in optimal cutting temperature compound (Tissue-Tek), and cut in 40 μm sections on a cryostat.

    Labeling:

    Article Title: Alteration of dorsal root ganglion P2X3 receptor expression and function following spinal nerve ligation in the rat.
    Article Snippet: One subtype of ATP-gated ion channel, the P2X3 receptor, is expressed primarily on peripheral sensory neurons.. While it is known that P2X3 receptors can participate in certain forms of nociceptive signaling, their involvement in neuropathic pain transmission is not known.. We have examined the expression and function of P2X3 receptors in a rat spinal nerve ligation model of neuropathic pain.

    Article Title: A long noncoding RNA contributes to neuropathic pain by silencing Kcna2 in primary afferent neurons
    Article Snippet: After being blocked for 1 h at 37°C in PBS containing 10% goat serum and 0.3% Triton X-100, the sections were incubated with rabbit anti-NF200 (1:500, Sigma-Aldrich, St. Louis, MO) 48 , rabbit anti-P2X3 (1:500, Neuromics, Edina, MN) 48 , Biotinylated IB4 (1:100, Sigma) 48 , rabbit anti-CGRP (1:500, EMD, Billerica, MA) 48 , mouse anti-NeuN (1:600, EMD) 48 , mouse anti-GFAP (1:500, Sigma) 48 , or mouse anti-OX-42 (1:400, Sigma) 48 overnight at 4°C.

    Article Title: The behavioral and neuroanatomical effects of IB4-saporin treatment in rat models of nociceptive and neuropathic pain.
    Article Snippet: One distinguishing feature of primary afferent neurons is their ability to bind the lectin IB4.. Previous work suggested that neurons in the inner part of lamina II (IIi), onto which IB4-positive sensory neurons project, facilitate nociceptive transmission following tissue or nerve injury.. Using an IB4-saporin conjugate (IB4-SAP), we examined the contribution of IB4-positive neurons to nociceptive processing in rats with and without nerve injury.

    Article Title: Low-Threshold Mechanosensitive VGLUT3-Lineage Sensory Neurons Mediate Spinal Inhibition of Itch by Touch
    Article Snippet: Skin sections from Vglut3-cre / TdTomato mice were blocked with 5% donkey serum and 0.2% Triton X-100 in PBS, then immunostained with rabbit anti-PGP9.5 (1:200; EMD Millipore), rabbit anti-calcitonin gene-related peptide (CGRP) (1:300; Peninsula Laboratories), rabbit anti-P2X3 (1:200; Neuromics), or rabbit anti-Neurofilament H (NFH) antibody (1:200, Sigma Millipore) at 4°C overnight, followed by incubation with the corresponding secondary antibody conjugated with AlexaFluor-488 (1:300) at 37°C for 30 min. For immunostaining of VGLUT3, skin was dissected from Vglut3-cre / TdTomato mice and frozen in optimal cutting temperature compound (Tissue-Tek), and cut in 40 μm sections on a cryostat.



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